Chapter 10: Sexual Reproduction and Genetics Chapter 10
Chapter 10: Sexual Reproduction and Genetics Chapter 10 Why does an organism look like it does? For a long time, people have observed that offspring look like their parents. Even before we knew about genes, people were breeding livestock to get certain
traits in the offspring. They knew that something caused babies to look like their parents. Most thought that traits of parents were blended in the offspring. 10.2: Mendelian Genetics Chapter 10 Gregor Mendel Gregor Mendel Father of Modern Genetics Austrian Monk
Work occurred in 1850s His work provides framework of what we know today Traits are distinguishing characteristics that are inherited. Mendel Laid the groundwork for genetics. Genetics is the study of biological inheritance patterns and variation. Gregor Mendel showed that traits are inherited as discrete units.
Many in Mendels day thought traits were blended. Mendel studied pea plants Why study pea plants? Reproduces quickly (14 days!) Self-pollinating or sexually reproduce Many varying traits Makes lots of offspring (100+!) Easy to grow Understood basic plant reproduction
Pollen & egg fused when fertilized One plant has & structures Most plants are self-fertilizing (purebred) Studied the traits of the plants Traits = characteristics Mendel cross fertilized plants with contrasting traits (tall and short) Mendels Garden
Seven Traits of Mendels Pea If allowed to self-pollinate, true-breeding plants produce offspring identical to themselves. Self-pollination pollen and ovum come from the same plant. True-breeding always produce offspring with the same trait.
He wondered what plants would look like if male sex cells in the pollen of one plant fertilize the egg cells on another plant = cross-pollination. Cross-pollination - using the pollen of one plant to fertilize the ovum of another plant Mendels Experiment He systematically cross- pollinated two different truebreeding plants called the
Parent generation (P). Mendel systematically cross-pollinated two different true- breeding plants called the Parent generation (P). His data revealed patterns of inheritance. Mendel made three key decisions in his experiments. use of purebred plants control over breeding observation of seven either-or traits
Cross Fertilizing taking pollen from one plant and fertilizing the egg of another Cross Fertilize = Offspring is called a Hybrid This caused odd outcomes Tall plant + short plant = tall plant
Mendel used pollen to fertilize selected pea plants. P generation crossed to produce F1 generation Mendel controlled the fertilization of his pea plants by removing the male parts, or stamens. He then fertilized the female part, or pistil, with pollen from a different pea plant.
Mendel allowed the resulting plants to self-pollinate. Among the F1 generation, all plants had purple flowers F1 plants are all heterozygous Among the F2 generation, some plants had purple flowers and some had white Mendel observed patterns in the first and second generations of his crosses. What patterns do you observe?
Mendel drew three important conclusions. Traits are inherited as discrete units. Organisms inherit two copies of each gene, one from each parent. The two copies segregate during gamete formation. The last two conclusions are called the law of segregation. purple white
Genes Genes encode proteins that produce a diverse range of traits. The same gene can have many versions. A gene is a piece of DNA that directs a cell to make a certain protein. Each gene has a locus, a specific position on a pair of homologous chromosomes. An allele is any alternative form of a gene
occurring at a specific locus on a chromosome. Each parent donates one allele for every gene. Homozygous describes two alleles that are the same at a specific locus. Heterozygous
describes two alleles that are different at a specific locus. Genotype vs. Phenotype Genes influence the development of traits. All of an organisms genetic material is called the genome. A genotype refers to the makeup of a specific set of genes.
A phenotype is the physical expression of a trait. Alleles can be represented using letters. A dominant allele is expressed as a phenotype when at least one allele is dominant. A recessive allele is expressed as a phenotype only when two copies are present.
Dominant alleles are represented by uppercase letters; recessive alleles by lowercase letters. Both homozygous dominant and heterozygous genotypes yield a dominant phenotype. Most traits occur in a range and do not follow simple dominant-recessive patterns.
Punnett squares Punnett squares illustrate genetic crosses. The Punnett square is a grid system for predicting all possible genotypes resulting from a cross. The axes represent the possible gametes of each parent. The boxes show the possible genotypes
the offspring. TheofPunnett square yields the ratio of possible genotypes and phenotypes. The inheritance of traits follows the rules of probability. Monohybrid Monohybrid crosses examine the inheritance of only one specific trait.
A testcross is a cross between an organism with an unknown genotype and an organism with the recessive phenotype. Probability Heredity patterns can be calculated with probability. Probability is the likelihood that something will happen. Probability predicts an average number of occurrences, not an exact number of occurrences. number of ways a specific event can occur
Probability number of total possible outcomes = Probability applies to random events such as meiosis and fertilization. Human Sex Determination What is the probability that a baby
will be a girl? A boy? Probabilities Predict Averages The larger the number of individuals, the closer the resulting offspring numbers will get to expected values. Probabilities do not
guarantee outcomes!!!! Tt Probability = number of ways a specific event can occur number of total possible outcomes x Tt
T T TT t Tt t Tt tt Tall: =75% Short: =25%
Punnett Squares Step by step how to guide Putting it together Alleles represented by letters Capital letters = dominant (T) Lowercase letters = recessive (t) Dominant letter goes before the recessive letter within Punnett square Two letters combined = trait TT, Tt, tt One from mom and one from dad
Homozygous = both letters same TT or tt Heterozygous = both letters differ Tt 6.5 Punnett Squares A diagram that shows all possible outcomes of a genetic cross Can be used to predict probabilities Phenotype an observable trait
Tall or short Genotype genetic make-up or combination of alleles TT Tt tt Initial Steps (for a monohybrid cross)
1. Identify the Parents being used in the cross Homozygous or heterozygous TT, Tt, tt? Most important step! 2. Segregate the alleles in each set of genes for each parent Show meiosis creating the haploid cells Parent: (P)Heterozygous
Tt x Tt Tall crossed with Heterozygous Segregati Tall on of alleles:
T Draw the Square: Place the parents: t T T
t T x t t T T
T t T T t t
T t t t Tt x Tt Whats next? T
t Genotypic T TT t Tt Ratio: Tt TT =1 Tt =2
tt =1 tt Phenotypic Ratio: Ratio of traits Tall = 3 Short =1
Ratios Ratio of allele combinations 1:2:1 3:1 Examples for you to work out! 1.
Tt x tt -be sure to show both types of ratios 2. Cross a homozygous tall plant with a short plant -be sure to show both types of ratios Dihybrid Crosses A dyhybrid cross involves two traits. Mendels dihybrid crosses with heterozygous plants
yielded a 9:3:3:1 phenotypic ratio. Mendels dihybrid crosses led to his second law, the law of independent assortment. The law of independent assortment states that allele pairs separate
independently of each Initial Steps (for a dihybrid cross) 1. Identify the Parents being used in the cross and figure out the combo of both traits. Homozygous or heterozygous TTGG, TTGg, TTgg, TtGG, TtGg, Ttgg, ttGG, ttGg, ttgg? Most important step! 2. Segregate the alleles in each set of genes
for each parent Show meiosis creating the haploid cells Heterozygous Tall, Heterozygous Green plant crossed with Heterozygous Tall, Heterozygous Green plant TtGg TG Tg tG tg
x TtGg x TG Tg tG tg TtGg Step 3 Set up and
complete the Punnett Square TG Tg tG x TtGg tg
TG T T G G T T G g T t G G T t G g Tg T T G T T T T G t gg t g gg g tG T t G G T t G g tt GG tt Gg
tg T t G g T t gg t t Gg t t gg Phenotypic Ratio Tall/ Green: Tall/ yellow:
Short/ Green: Short /Yellow: Phenotypic Ratio: 9 3 3 9:3:3:1
1 Examples for you to work out! 1. Cross a heterozygous tall, yellow plant with a homozygous tall, heterozygous green plant. 2. Cross a heterozygous tall, yellow plant
with a short, heterozygous green plant. Summary of Mendels Work Traits are determined by genes which are passed from parent to offspring. Some forms of a genes may be dominant and some recessive for a given trait. Most sexually reproducing organisms have 2 alleles for a gene that separate when eggs and sperm are formed. Alleles for different genes can segregate
independently of one another. 10.3 Gene Linkage and Polyploidy Chapter 10 Gene Linkage and Mapping KEY CONCEPT: Genes can be mapped to specific locations on chromosomes. Gene linkage was explained through fruit flies. Morgan found that linked traits are on the same
chromosome. Chromosomes, not genes, assort independently during meiosis. Wild type Mutant Gene Linkage Linked genes are not inherited together every time. Chromosomes exchange homologous genes
during meiosis. Genes located close together on a chromosome are likely to be inherited together. http://www.csun.edu/~cmalone/ pdf360/Ch06-1chi%202pt.pdf Linkage Maps
Linkage maps estimate distances between genes. The closer together two genes are, the more likely they will be inherited together. Cross-over frequencies are related to distances between genes. Linkage maps show the relative locations of genes. Crossing Over frequencies Cross-over frequencies can be converted into map
units. gene A and gene B cross over 6.0 percent of the time gene B and gene C cross over 12.5 percent of the time gene A and gene C cross over 18.5 percent of the time
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