National 4, National 5 Biology Microbiology Activities Cauliflower

National 4, National 5 Biology Microbiology Activities Cauliflower cloning: www.saps.org.uk Cauliflower cloning 3. Collect a small mini-floret of cauliflower and place

in a Petri dish. 4. Using a scalpel, cut the mini-floret into microflorets small 3-5 mm pieces (explants) 5. Transfer the explants to the jar of SDICN. Put the lid on and swirl the jar for 5 s. Replace forceps. 6. Swirl the jar gently for 5 s every 2-3 minutes for 15 minutes. (Do this whilst carrying out the next activity)

Cauliflower cloning 7. Take the lid off the vial of growth medium and place face-down on a clean tile. 8. Using sterile forceps, pick an explant from

the SDICN and place into the agar. Press the stalk into the medium slightly. Replace the lid. Cauliflower cloning 9. Label the vial with

your initials and the date. 10. Incubate in a warm lab near window or light bank. Examine / photograph at regular intervals.

Cauliflower explant after 10 weeks incubation. Knowledge and understanding Basic aseptic technique Basic microbiological techniques

Media appropriate for type of microorganism (growth requirements) Temperature requirements for growth of micoorganisms The importance of aseptic technique (relevant applications of biology) Microbiology

Cell / tissue culture animals, plants Medical /clinical prevention of disease, infection Forensic e.g. DNA analysis Good Manufacturing Practice (GMP) food, pharmaceuticals etc. Culturing human cells HeLa cells

Science content Scientific method Ethical issues http://archive.org/details/AdamCurtisTheWayofAllFlesh http://www.bbc.co.uk/blogs/adamcurtis/posts/ the_undead_henrietta_lacks_and

Safety / Guidance / Resources http://www.sserc.org.uk/ SSERC Code of Practice SSERC microbiology techniques cards Safety / Guidance / Resources Microbiology in Schools Advisory Service (MiSAC)

http://www.misac.org.uk/ Society for General Microbiology (SGM) http://www.microbiologyonline.org.uk/ National Centre for Biotechnology Education (NCBE) http://www.ncbe.reading.ac.uk/

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